The custom software (Bioptigen, Durham, NC, USA) enables imaging, analyzing, and archiving of the acquired data. The handheld probe has a focus adjustment system and a diopter scale bar with a range of +10 to −12 D. A reference arm position adjuster with digital readout is also included into the OCT system. The OCT unit is mounted on a trolley cart to increase the mobility in the operating room, as well as a screen, keyboard, and printer. The SD-OCT engine is connected via a 1.3-m flexible cable to the imaging handpiece that is used by the operator. 1 (BioptigenEnvisu 2015) Bioptigen SDOIS is a noncontact, portable, handheld SD-OCT system. Their Bioptigen Envisu Spectral Domain Ophthalmic Imaging System (SDOIS) was developed to capture, process, display, and save depth-resolved images of ocular tissue microstructures based on a spectral domain OCT source Bioptigen Inc. A forward-imaging probe would be most desirable to examine the inner structures of a hollow organ such as the eye, in order to evaluate the retina. By using 2D imaging with a combination of probe rotation or linear movement, three dimensional (3D) images can be obtained. Usually, two-dimensional (2D) data imaging is provided by forward-imaging probes. A forward-imaging OCT probe, with relatively large diameter, has also been used to image bladders (Feldchtein et al. in 2000 for interstitial imaging of different organs (Li et al. The first side imaging, 27-gage needle probe OCT, was designed by Li et al. Those OCT probes are being successfully utilized in imaging of the esophagus, coronary arteries, etc., with a lateral resolution up to 10 μm. OCT side-scanning probes with different sizes have been designed to examine tissues within tubular structures. The three main probe parameters are field of view, scanning range, and scanning rate. According to the direction of the optical scanning mechanism, the OCT probes can be divided into two main types: side-imaging probes and forward-imaging probes (Sun et al.
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